本发明公开了一种手性均一且可控的超分子水凝胶因子的制备方法；包括如下步骤：S1、将对苯基酰氯或异氰酸酯与苯丙氨酸甲酯盐酸盐溶于有机溶剂，碱性环境下生成二酯中间产物；S2、将中间产物脱甲酯后盐酸调pH值，产生白色沉淀，过滤、冲洗、真空干燥得二酸中间产物；S3、将中间产物与二甘醇胺或3-氨基-1,2-丙二醇在酰胺缩合剂作用下进行缩合，经分离纯化后即得超分子水凝胶因子。与现有技术相比，本发明的手性均一且可控的超分子水凝胶因子的制备方法工艺流程短、操作简单、反应条件温和、反应产率高、纯度高，克服了现有技术制备过程复杂且不易控制的缺点，从而可以大批量工业生产，适用于商业化细胞三维培养支架材料等。

The invention discloes a preparation method for chiral uniform and controllable supramolecular hydrogel factor; It comprises the following steps: S1, p-phenylacyl chloride or isocyanate and phenylalanine methyl ester hydrochloride are dissolved in an organic solvent to generate diester intermediate products in an alkaline environment; S2. The intermediate product was demethylated and pH was adjusted with hydrochloric acid to produce a white precipitate. The intermediate product was filtered, rinsed, and dried under vacuum to obtain diacid. S3, the intermediate was mixed with diethylene glycol amine or 3- Amino group- 1, 2-The supramolecular hydrogel factor was obtained by condensation of propylene glycol under the action of amide condensation mixture and separation and purification. Compared with the prior art, the preparation method of the supramolecular hydrogel factor with uniform chirality and controllability of the present invention has the advantages of short process, simple operation, mild reaction conditions, high reaction yield and high purity, which overcomes the shortcomings of the prior art of complex and difficult to control preparation process, so that it can be produced in large quantities and is suitable for commercial three-dimensional cell culture scaffold materials.